Regulation of promoter occupancy during activation of cryptobiotic embryos from the crustacean Artemia franciscana

Regulation of promoter occupancy during activation of cryptobiotic embryos from the crustacean Artemia franciscana.

Artemia franciscana embryos can suspend their development and metabolism at the gastrula stage to enter a state of cryptobiosis, forming cysts. Embryonic development and metabolism can be resumed under favorable environmental conditions to give rise to free-swimming larvae or nauplii. The mechanisms that mediate these processes are not completely known. Here, we report our studies of the mechan...

Transcriptional regulation during the activation and development of Artemia franciscana encysted embryos.

Early embryos of the crustacean Artemia franciscana (brine shrimp) can get into cryptobiosis under adverse environmental conditions. Under these circumstances, the embryo arrests all metabolic activities, gets dehydrated and is surrounded by a hard shell. These cysts are viable for long periods and can be activated when environmental conditions are favorable. Once the cyst is activated, the emb...

Metabolic downregulation during diapause in embryos of Artemia franciscana

Gene expression in diapause-destined embryos of the crustacean, Artemia franciscana

Diapause-destined embryos of the crustacean Artemia franciscana cease development as gastrulae, encyst, and enter a resting stage characterized by greatly reduced metabolic activity and extreme stress resistance. To better understand diapause induction and maintenance in Artemia embryos gene expression was analyzed by subtractive hybridization at two days post-fertilization, a time early in thi...

Characterization of a functional serum response element in the Actin403 gene promoter from the crustacean Artemia franciscana.

The serum response factor (SRF) activates expression of several genes in response to growth factors present in serum. SRF also regulates the expression of tissue-specific genes, including those in vertebrate muscles. An SRF-binding site (CArG box) present in the Artemia franciscana Actin403 promoter was shown to be necessary for transcriptional activity in cultured cells from Drosophila melanog...